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Stone, Daniel J; Macias-Contreras, Miguel; Crist, Shaun M; Bucag, Christelle_F T; Seo, Gwimoon; Zhu, Lei (, Organic & Biomolecular Chemistry)The sequential biochemical (SNAP-tag) and chemical (chelation-assisted copper-catalyzed azide–alkyne cycloaddition) reactions are applied in membrane protein labeling on live cells. The second, chemical step is rapid (within 1 minute) without any ill-effect to the labeled cells.more » « less
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